Mouse Perfusion Equipment

ELISA Detection Of Endogenous Serum Albumin In The Mouse ...
Equipment . 1. Scissors (Fine Science Tools, catalog number: 14002- 14) recovered for 72 h prior to perfusion. Figure 1.Procedure of cerebral cold injury. A copper rod (10 cm length, 4 mm diameter) B. Mouse perfusion 1. Make experimental manipulations toreproduce brain insults ... Fetch Document

Adult Mouse Transcardial Perfusion MBL 2011
Transcardial Perfusion 2011 BIE Summer Course John V. Brigande, PhD 1 Transcardial Perfusion of Mice Experimental Goal: To chemically fix the adult mouse inner ear by transcardial perfusion Rationale: Transcardial perfusion with aldehyde-based fixatives is generally considered to be an effective paradigm to preserve the soft issues of the entire inner ear. ... View This Document

Isolation Of Highly Pure Primary Mouse Alveolar Epithelial ...
Equipment . 1. Fine-tipped small scissors (Roboz surgical instrument company, catalog number: RS-5910) Cardiac perfusion of mouse lungs via the right ventricle . C. Tracheal cannulation 1. Flip the dissection board such that the head of the mouse is closer to you. ... View Full Source

Euthanasia - Welcome To URMC
Equipment. You should specifically indicate who will be performing euthanasia in the Associates section of the protocol. or perfusion of a histological fixative via the major blood vessels. Please see the UCAR policy on Euthanasia for Rodent Embryos, Fetuses and Neonates on the UCAR ... Read More

1. Protocols
Stereotaxic injection of vectors or molecules into the mouse brain Tamoxifen Gavaging Protocol Transcardial perfusion (mouse) Vibratome Sectioning Viral Injection Surgery Wholemount Staining and Ependymal Flow € Bromodeoxyuridine (BrdU) Labelling Contents Instructions Notes Equipment Consumables and Reagents References ... Retrieve Here

Fly Ash Grouting Stabilization Of Embankment - YouTube
The repair, by adding the surface active lime fly ash mixed slurry agent for blanket type grouting, on area of 2673m2 perfusion for about 460m3 mixed slurry, the embankment has been stable and ... View Video

Perfusion-Fixation Of Adult Rat Brain
Perfusion-Fixation of Adult Rat Brain K. Harris Lab, Center for Learning and Memory, The University of Texas at Austin Our standard perfusion fixative, 2% formaldehyde + 2.5% glutaraldehyde, is shown left. Equipment/Supplies Personnel Protective Equipment: Eye protection, face shield ... Fetch This Document

Cardiac Puncture Blood Collection (Terminal Procedure) SOP # ...
Cardiac Puncture Blood Collection (Terminal Procedure) SOP #PRO-002 Equipment required: • Mouse: 23-25 gauge needle and 1-3 ml syringe • Rat: 20-25 gauge needle and 10-20 ml syringe • Anesthesia Technique: 1. Anesthetize animal. (Surgical plane of anesthesia is required!) 2. Test for reaction by corneal reflex and toe pinch. 3. ... Access Doc

Immunohistochemistry In The Heart: A Protocol Manual
Immunohistochemistry in the Heart: A Protocol Manual By: Jacque A. Keele and Mark M. Stayton Department of Molecular Biology, University of Wyoming, Figure 5: Naïve expression of N-cadherin (1:2 dilution) of mouse heart using the Olympus microscope 40X magnification. Changing the exposure ... View This Document

BLF22 Surgical Protocol - Transonic.com
BLF22 Surgical Protocol APPLICATION BASICS Stroke Model: Middle Cerebral Artery Occlusion equipment. Founded in 1983, Transonic sells “gold standard” transit-time ultrasound BLF22 Acute MCA Occlusion Model Cerebral Perfusion in Mouse Author: Transonic Systems Inc. Subject: ... Return Doc

Recommended Methods Of Euthanasia: Mouse Rat
Recommended Methods of Euthanasia: Mouse / Rat Species Method Description Mouse / Rat / Hamster / Guinea Pig cylinder or tank, pressure reducing regulator, and flow meter, or equivalent equipment. CO2 flow will be maintained for at least one minute following respiratory arrest. ... Access Content

Perfusion Techniques - Usf.edu
Perfusion Techniques • Perfusion is done to fix tissues. In humans this is known as embalming. • To reduce the amount of fixative needed and allow for good perfusions, you may be asked to Position the mouse so that ... Read Content

T400-Series Surgical Protocol - Transonic
T400-Series Surgical Protocol Volume Flow Pressure from the retrograde perfusion causes the aortic valve to close and forces the solution into the coronary circulation, draining via the coronary sinus into the MOUSE RAT FLOW RANGE (ml/min) INLINE FLOWSENSOR FLOW RANGE (ml/min) INLINE ... Get Document

High-Density Lipoprotein Stimulates Myocardial Perfusion In Vivo
Heart weight was measured after excision for each individual mouse. The mean heart weights were identical in the WT and S1P 3 Technical & Scientific Equipment GmbH). Statistical Analysis Perfusion values are expressed as mean SEM. Two-way ANOVA perfusion, although it is not an absolute ... Access Content

Perfusion Two™ - Meyer Instruments
Tops to attach to Perfusion One, and Luer endings to attach gavage needles (included). Mice need the one with lower internal volume, due to the much lower flow rate through a mouse. See useage notes for rationale. Tubing from the lids connects to 3 numbered ports on the side of the cover. ... Read Document

SUMMARY OF SAFETY AND PROBABLE BENEFIT I. GENERAL INFORMATION
SUMMARY OF SAFETY AND PROBABLE BENEFIT . I. GENERAL INFORMATION. Device Generic Name: Ex Vivo Lung Perfusion (EVLP) Device Trade Name: XVIVO Perfusion System (XPSTM) with STEEN Solution™ Perfusate . evaluation of mouse fibroblast cell culture. ... Read Content

The Use Of Liver Microsome In-Vitro Methods To Study Toxicant ...
Metabolism and Predict Species Efficacy Katherine E. Horak, Chad R. Wermager, and Thomas M. Primus samples from wildlife of interest would likely be harvested in the field without access to laboratory perfusion equipment. Therefore, ... Fetch Document

Isolated Heart ISOLATED PERFUSED HEART Perfusion Systems
Isolated Heart Perfusion Systems for mouse to rabbit animal models Cardioplegia Refractory Period Tissue Engineering Contractility Studies signal conditioning equipment, and acquisition & analysis software to provide a superior solution for virtually any study. ... View Document

Isolated Perfused Lung Systems Guide - Harvard Apparatus
Perfusion Systems for small to large animal models Hypoxic Vasoconstriction respiratory mechanics and pulmonary physiology systems tailored for specific species from mouse to pig. signal conditioning equipment, and acquisition & analysis software, providing a superior solution for ... View This Document

Agitation, Aeration And perfusion Modules For Cell Culture ...
Agitation, aeration and perfusion modules for cell culture bioreactors Christel Fenge, Cornelia Klein, Carsten Heuer, Ursula Siegel and Elisabeth Fraune aeration systems and perfusion equipment. As a result a flexible bioreactor system A mouse-mouse hybridoma cell line (ATCC HB 124) ... Return Document

Single-photon Emission Computed Tomography - Wikipedia
Single-photon emission computed tomography (SPECT, gated myocardial SPECT can be used to obtain quantitative information about myocardial perfusion, thickness, and the gamma scanning equipment is less expensive as well. ... Read Article

New Anti-arrhythmics To Vanquish Sudden Cardiac Death Or AFIb ...
New Anti-arrhythmics To Vanquish Sudden Cardiac Death or AFIb: KOR Therapies Video 2 This lead drug was added to the perfusion solution at ~9:30 kinase ζ inhibits ventricular ... View Video